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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NB100-507 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-507, RRID:AB_10002919
- Product name
- Mouse Monoclonal KAT3B/p300 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Antigen sequence
The epitope recognized is found bet
ween amino acids 1868-1921.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer.
Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.
Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.
Polyomavirus large T antigen binds the transcriptional coactivator protein p300.
Polyomavirus large T antigen binds the transcriptional coactivator protein p300.
Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
Association of p300 and CBP with simian virus 40 large T antigen.
Association of p300 and CBP with simian virus 40 large T antigen.
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Paladino D, Yue P, Furuya H, Acoba J, Rosser CJ, Turkson J
Oncotarget 2016 Feb 9;7(6):7253-67
Oncotarget 2016 Feb 9;7(6):7253-67
Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.
Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM
Genes & development 1999 Jan 1;13(1):64-75
Genes & development 1999 Jan 1;13(1):64-75
Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.
Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM
Genes & development 1999 Jan 1;13(1):64-75
Genes & development 1999 Jan 1;13(1):64-75
Polyomavirus large T antigen binds the transcriptional coactivator protein p300.
Nemethova M, Wintersberger E
Journal of virology 1999 Feb;73(2):1734-9
Journal of virology 1999 Feb;73(2):1734-9
Polyomavirus large T antigen binds the transcriptional coactivator protein p300.
Nemethova M, Wintersberger E
Journal of virology 1999 Feb;73(2):1734-9
Journal of virology 1999 Feb;73(2):1734-9
Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
Eckner R, Yao TP, Oldread E, Livingston DM
Genes & development 1996 Oct 1;10(19):2478-90
Genes & development 1996 Oct 1;10(19):2478-90
Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
Eckner R, Yao TP, Oldread E, Livingston DM
Genes & development 1996 Oct 1;10(19):2478-90
Genes & development 1996 Oct 1;10(19):2478-90
Association of p300 and CBP with simian virus 40 large T antigen.
Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA
Molecular and cellular biology 1996 Jul;16(7):3454-64
Molecular and cellular biology 1996 Jul;16(7):3454-64
Association of p300 and CBP with simian virus 40 large T antigen.
Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA
Molecular and cellular biology 1996 Jul;16(7):3454-64
Molecular and cellular biology 1996 Jul;16(7):3454-64
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM
Genes & development 1994 Apr 15;8(8):869-84
Genes & development 1994 Apr 15;8(8):869-84
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB, Livingston DM
Genes & development 1994 Apr 15;8(8):869-84
Genes & development 1994 Apr 15;8(8):869-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW128) antibody NB100-507 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
