Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-2949-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD294 (CRTH2) Monoclonal Antibody (BM16), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This BM16 monoclonal antibody reacts with human CD294, which is also known as G protein-coupled receptor-44 (GPR-44) or chemoattractant receptor expressed on Th2 cells (CRTH2). CD294 is expressed on Th2 cells, some CD8+ cells, eosinophils, and basophils. Moreover, CD294 binds prostaglandin D2, which promotes leukocyte chemotaxis during allergic responses. CD294 has been reported to mediate prostaglandin D2-mediated suppression of apoptosis of Th2 cells by activating the PI3K pathway. Applications Reported: This BM16 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BM16 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- BM16
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Novel function of CRTH2 in preventing apoptosis of human Th2 cells through activation of the phosphatidylinositol 3-kinase pathway.
GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells.
Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2.
Xue L, Barrow A, Pettipher R
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7580-6
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7580-6
GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells.
De Fanis U, Mori F, Kurnat RJ, Lee WK, Bova M, Adkinson NF, Casolaro V
Blood 2007 May 15;109(10):4343-50
Blood 2007 May 15;109(10):4343-50
Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2.
Hirai H, Tanaka K, Yoshie O, Ogawa K, Kenmotsu K, Takamori Y, Ichimasa M, Sugamura K, Nakamura M, Takano S, Nagata K
The Journal of experimental medicine 2001 Jan 15;193(2):255-61
The Journal of experimental medicine 2001 Jan 15;193(2):255-61
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or Anti-Human CD294 (CRTH2) APC (right). Cells in the lymphocyte gate were used for analysis.