BS-15430R

antibody from Invitrogen Antibodies

Targeting: HCST DAP10, DKFZP586C1522, KAP10, PIK3AP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-15430R

Provider

Invitrogen Antibodies

Product name

HCST Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PANC-1 lysates probed with HCST Polyclonal Antibody, Unconjugated (bs-15430R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HCST Polyclonal Antibody, Unconjugated (bs-15430R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.