Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-55403 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55403, RRID:AB_2117808
- Product name
- Goat Polyclonal DAP10/HCST Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
RSPAQEDGKVYIN
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells.
Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ
Nature immunology 2006 May;7(5):524-32
Nature immunology 2006 May;7(5):524-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HCST Antibody [NB100-55403] - (0.2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.