Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [2]
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Validation data
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- Product number
- PA5-77752 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references MiR-203-3p inhibits the oxidative stress, inflammatory responses and apoptosis of mice podocytes induced by high glucose through regulating Sema3A expression.
Chen J, Xu Q, Zhang W, Zhen Y, Cheng F, Hua G, Lan J, Tu C
Open life sciences 2020;15(1):939-950
Open life sciences 2020;15(1):939-950
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat brain mebranes (lanes 2 and 4) with SEMA3A polyclonal antibody (Product # PA5-77752) using a dilution of 1:400.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat brain mebranes (lanes 2 and 4) with SEMA3A polyclonal antibody (Product # PA5-77752) using a dilution of 1:400.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat brain mebranes (lanes 2 and 4) with SEMA3A polyclonal antibody (Product # PA5-77752) using a dilution of 1:400.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Sema3A expression was increased in podocytes exposed to HG. (a and b) The WB analysis was performed to assess the protein level of Sema3A in different concentrations of glucose (0, 10, 20, 30 and 40 mM) or time points (0, 12, 24 and 48 h) of HG treatment. (c) The effect of miR-203-3p expression on Sema3A protein level was detected by the WB analysis. * P < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 MiR-203-3p and Sema3A regulated the oxidative stress of HG-induced podocytes. The HG-induced podocytes were transfected with miR-con, miR-203-3p, miR-203-3p + pcDNA or miR-203-3p + Sema3A. (a) The protein level of Sema3A was determined by the WB analysis. (b-d) ROS production, MDA level and SOD activity were measured by the corresponding ELISA kits. * P < 0.05.