M02193

antibody from Boster Biological Technology

Targeting: ADK AK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

M02193

Provider

Boster Biological Technology

Product name

Anti-ADK Antibody Picoband™ (monoclonal, 7F4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for ADK detection. Tested with WB, IHC-P, FCM in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7F4

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of ADK using anti-ADK antibody (M02193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADK antigen affinity purified monoclonal antibody (Catalog # M02193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ADK at approximately 45KD. The expected band size for ADK is at 45KD.

Additional image