PA5-120323

antibody from Invitrogen Antibodies

Targeting: ADK AK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120323

Provider

Invitrogen Antibodies

Product name

Adenosine Kinase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: LO2, A-431, Mouse testis, Mouse kidney, Mouse spleen, Rat liver. The target is usually found in the following locations: Cytoplasm, Nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.79 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Adenosine Kinase in various cell lines using a Adenosine Kinase Polyclonal antibody (Product # PA5-120323) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 5s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adenosine Kinase in C6 cells using a Adenosine Kinase Polyclonal antibody (Product # PA5-120323). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adenosine Kinase in C6 cells using a Adenosine Kinase Polyclonal antibody (Product # PA5-120323). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Adenosine Kinase in C6 cells using a Adenosine Kinase Polyclonal antibody (Product # PA5-120323). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Adenosine Kinase in human placenta using a Polyclonal antibody (Product # PA5-120323) at a dilution of 1:350. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Adenosine Kinase in human placenta using a Polyclonal antibody (Product # PA5-120323) at a dilution of 1:350. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Adenosine Kinase in human placenta using a Polyclonal antibody (Product # PA5-120323) at a dilution of 1:350. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..