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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA809917 - Provider product page
- Provider
- OriGene
- Product name
- SEMA3D mouse monoclonal antibody,clone OTI2H4
- Antibody type
- Monoclonal
- Description
- SEMA3D mouse monoclonal antibody,clone OTI2H4
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SEMA3D
- Isotype
- IgG
- Antibody clone number
- OTI2H4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEMA3D (RC216032, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEMA3D.(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-SEMA3D mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA809917)(1:500)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-SEMA3D mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA809917)(1:500)
- Validation comment
- IHC
