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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-27774 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPY Monoclonal Antibody (GT6512)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT6512
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPY in A) 293T whole cell lysate, B) whole cell lysate of human PPY-transfected 293T cells using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with PPY monoclonal antibody (Product # MA5-27774) using a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PPY Monoclonal Antibody (GT6512) detects PPY protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PPY-transfected 293T cells.15 % SDS-PAGE. PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PPY was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PPY was performed in paraffin-embedded mouse pancreas tissue using PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) at a dilution of 1:500.
