Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
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Validation data
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- Product number
- ADI-905-746-100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-905-746-100, RRID:AB_2038805
- Product name
- Angiotensin II receptor AT2, pAb
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide affinity purified. Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Submitted references Immunohistochemical detection of angiotensin II receptors in mouse cerebellum and adrenal gland using "in vivo cryotechnique".
Huang Z, Ohno N, Terada N, Saitoh Y, Chen J, Ohno S
Histochemistry and cell biology 2013 Oct;140(4):477-90
Histochemistry and cell biology 2013 Oct;140(4):477-90
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis: MW marker (1) and 20µg rat kidney membrane extract (2) probed with Angiotensin II Receptor (AT2) pAb at 6µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- Immunohistochemistry analysis: Rat brain nucleus tractus solitarius (NTS) fixed in 100% methanol, then stained with Angiotensin II Receptor (AT2) pAb at 1:1000.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Membrane ELISA: Lewis rat kidney membranes (5µg/well) were treated with 1µM concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT2) pAb (1:250 to 1:2000 of a 1µg/µL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).