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Flow cytometry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB3648 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Complement Component C5aR1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P21730- Isotype
- IgG
- Antibody clone number
- 347214
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references C5a and C5aR are elevated in joints of rheumatoid and psoriatic arthritis patients, and C5aR blockade attenuates leukocyte migration to synovial fluid.
Hornum L, Hansen AJ, Tornehave D, Fjording MS, Colmenero P, Wätjen IF, Søe Nielsen NH, Bliddal H, Bartels EM
PloS one 2017;12(12):e0189017
PloS one 2017;12(12):e0189017
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- N-Acetyl-beta-D-Glucosa-minidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a R1 Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta-D-glucosa-minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta-D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2-10 µg/mL in the presence of cytochalasin-B.
