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LearnMAB150-100
antibody from R&D Systems
Targeting: CCR2
CC-CKR-2, CD192, CKR2, CMKBR2, FLJ78302, MCP-1-R
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB150-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_001116868- Isotype
- IgG
- Antibody clone number
- 48607
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys197 and by transmembrane protein aptamers that target it for lysosomal degradation.
CSF-1 regulates the function of monocytes in Crohn's disease patients in remission.
Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas.
Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression.
Evidence for chemokine synergy during neutrophil migration in ARDS.
Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity.
HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
PC3-secreted microprotein is a novel chemoattractant protein and functions as a high-affinity ligand for CC chemokine receptor 2.
Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Changes of peripheral TGF-β1 depend on monocytes-derived macrophages in Huntington disease.
Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Comparison of gene expression profiles between human and mouse monocyte subsets.
Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
A CCR2-V64I polymorphism affects stability of CCR2A isoform.
Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Petti LM, Marlatt SA, Luo Y, Scheideman EH, Shelar A, DiMaio D
The Journal of biological chemistry 2018 Jun 8;293(23):8787-8801
The Journal of biological chemistry 2018 Jun 8;293(23):8787-8801
CSF-1 regulates the function of monocytes in Crohn's disease patients in remission.
Nieto JC, Zamora C, Cantó E, Garcia-Planella E, Gordillo J, Ortiz MA, Juárez C, Vidal S
Scientific reports 2017 Mar 7;7(1):92
Scientific reports 2017 Mar 7;7(1):92
Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas.
Przybyl J, Kowalewska M, Quattrone A, Dewaele B, Vanspauwen V, Varma S, Vennam S, Newman AM, Swierniak M, Bakuła-Zalewska E, Siedlecki JA, Bidzinski M, Cools J, van de Rijn M, Debiec-Rychter M
JCI insight 2017 Jun 2;2(11)
JCI insight 2017 Jun 2;2(11)
Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression.
Sebastiani G, Ventriglia G, Stabilini A, Socci C, Morsiani C, Laurenzi A, Nigi L, Formichi C, Mfarrej B, Petrelli A, Fousteri G, Brusko TM, Dotta F, Battaglia M
Scientific reports 2017 Jul 31;7(1):6897
Scientific reports 2017 Jul 31;7(1):6897
Evidence for chemokine synergy during neutrophil migration in ARDS.
Williams AE, José RJ, Mercer PF, Brealey D, Parekh D, Thickett DR, O'Kane C, McAuley DF, Chambers RC
Thorax 2017 Jan;72(1):66-73
Thorax 2017 Jan;72(1):66-73
Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity.
Maghazachi AA, Sand KL, Al-Jaderi Z
Frontiers in immunology 2016;7:437
Frontiers in immunology 2016;7:437
HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
Chandrasekaran P, Moore V, Buckley M, Spurrier J, Kehrl JH, Venkatesan S
PloS one 2014;9(1):e86998
PloS one 2014;9(1):e86998
PC3-secreted microprotein is a novel chemoattractant protein and functions as a high-affinity ligand for CC chemokine receptor 2.
Pei X, Sun Q, Zhang Y, Wang P, Peng X, Guo C, Xu E, Zheng Y, Mo X, Ma J, Chen D, Zhang Y, Zhang Y, Song Q, Guo S, Shi T, Zhang Z, Ma D, Wang Y
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1878-86
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1878-86
Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
Manley NC, Caso JR, Works MG, Cutler AB, Zemlyak I, Sun G, Munhoz CD, Chang S, Sorrells SF, Ermini FV, Decker JH, Bertrand AA, Dinkel KM, Steinberg GK, Sapolsky RM
PloS one 2013;8(4):e61789
PloS one 2013;8(4):e61789
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Duluc D, Gannevat J, Anguiano E, Zurawski S, Carley M, Boreham M, Stecher J, Dullaers M, Banchereau J, Oh S
Mucosal immunology 2013 May;6(3):626-38
Mucosal immunology 2013 May;6(3):626-38
Changes of peripheral TGF-β1 depend on monocytes-derived macrophages in Huntington disease.
Di Pardo A, Alberti S, Maglione V, Amico E, Cortes EP, Elifani F, Battaglia G, Busceti CL, Nicoletti F, Vonsattel JP, Squitieri F
Molecular brain 2013 Dec 13;6:55
Molecular brain 2013 Dec 13;6:55
Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
International journal of cancer 2010 Mar 1;126(5):1095-108
International journal of cancer 2010 Mar 1;126(5):1095-108
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
International journal of cancer 2010 Jul 15;127(2):332-44
International journal of cancer 2010 Jul 15;127(2):332-44
Comparison of gene expression profiles between human and mouse monocyte subsets.
Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht AJ, Ziegler-Heitbrock L, Randolph GJ
Blood 2010 Jan 21;115(3):e10-9
Blood 2010 Jan 21;115(3):e10-9
Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1270-7
Journal of immunology (Baltimore, Md. : 1950) 2009 Feb 1;182(3):1270-7
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP 3rd, Zisman DA, Belperio JA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
Molecular pharmacology 2008 Aug;74(2):485-95
Molecular pharmacology 2008 Aug;74(2):485-95
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
The American journal of pathology 2007 Dec;171(6):1789-99
The American journal of pathology 2007 Dec;171(6):1789-99
HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
Eugenin EA, Dyer G, Calderon TM, Berman JW
Glia 2005 Mar;49(4):501-10
Glia 2005 Mar;49(4):501-10
A CCR2-V64I polymorphism affects stability of CCR2A isoform.
Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T
AIDS (London, England) 2004 Mar 26;18(5):729-38
AIDS (London, England) 2004 Mar 26;18(5):729-38
Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
Journal of immunology (Baltimore, Md. : 1950) 2004 Jun 1;172(11):7031-42
Journal of immunology (Baltimore, Md. : 1950) 2004 Jun 1;172(11):7031-42
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Lim HW, Hillsamer P, Kim CH
The Journal of clinical investigation 2004 Dec;114(11):1640-9
The Journal of clinical investigation 2004 Dec;114(11):1640-9
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood 2004 Apr 1;103(7):2806-8
Blood 2004 Apr 1;103(7):2806-8
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Campbell JJ, Qin S, Unutmaz D, Soler D, Murphy KE, Hodge MR, Wu L, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
The Journal of biological chemistry 1999 Apr 2;274(14):9617-26
The Journal of biological chemistry 1999 Apr 2;274(14):9617-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCR2 in Human Tonsil. CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
