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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX104745 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104745, RRID:AB_1241440
- Product name
- VDAC2 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Cho CK, Smith CR, Diamandis EP
Journal of proteome research 2010 Jul 2;9(7):3574-82
Journal of proteome research 2010 Jul 2;9(7):3574-82
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Supportive validation
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- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse liver 12% SDS PAGE GTX104745 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody [C2C3], C-term detects VDAC2 protein by western blot analysis.A. 30 ?g A549 whole cell lysate/extract12 % SDS-PAGEVDAC2 antibody [C2C3], C-term (GTX104745) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody detects VDAC2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and blotted with VDAC2 antibody (GTX104745) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using VDAC2(GTX104745) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody [C2C3], C-term immunoprecipitates VDAC2 protein in IP experiments.IP samples: HepG2 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of VDAC2 protein by 4 £gg VDAC2 antibody [C2C3], C-term (GTX104745)10 % SDS-PAGEThe immunoprecipitated VDAC2 protein was detected by VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded normal liver. VDAC2 antibody [C2C3], C-term (GTX104745) dilution: 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VDAC2 antibody [C2C3], C-term detects VDAC2 protein at mitochondria in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. VDAC2 antibody [C2C3], C-term (GTX104745) diluted at 1:500.
