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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB182-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCR5.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51681- Isotype
- IgG
- Antibody clone number
- 45531
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCR5 structural plasticity shapes HIV-1 phenotypic properties.
Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
The HIV co-receptor CCR5 regulates osteoclast function.
The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.
CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association.
Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1.
Functional diversity of human vaginal APC subsets in directing T-cell responses.
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Colin P, Zhou Z, Staropoli I, Garcia-Perez J, Gasser R, Armani-Tourret M, Benureau Y, Gonzalez N, Jin J, Connell BJ, Raymond S, Delobel P, Izopet J, Lortat-Jacob H, Alcami J, Arenzana-Seisdedos F, Brelot A, Lagane B
PLoS pathogens 2018 Dec;14(12):e1007432
PLoS pathogens 2018 Dec;14(12):e1007432
Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
Wu WL, Grotefend CR, Tsai MT, Wang YL, Radic V, Eoh H, Huang IC
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117
The HIV co-receptor CCR5 regulates osteoclast function.
Lee JW, Hoshino A, Inoue K, Saitou T, Uehara S, Kobayashi Y, Ueha S, Matsushima K, Yamaguchi A, Imai Y, Iimura T
Nature communications 2017 Dec 20;8(1):2226
Nature communications 2017 Dec 20;8(1):2226
The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.
Ward ST, Li KK, Hepburn E, Weston CJ, Curbishley SM, Reynolds GM, Hejmadi RK, Bicknell R, Eksteen B, Ismail T, Rot A, Adams DH
British journal of cancer 2015 Jan 20;112(2):319-28
British journal of cancer 2015 Jan 20;112(2):319-28
CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association.
Flegler AJ, Cianci GC, Hope TJ
PloS one 2014;9(2):e89056
PloS one 2014;9(2):e89056
Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1.
Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y
Virology 2014 Mar;452-453:117-24
Virology 2014 Mar;452-453:117-24
Functional diversity of human vaginal APC subsets in directing T-cell responses.
Duluc D, Gannevat J, Anguiano E, Zurawski S, Carley M, Boreham M, Stecher J, Dullaers M, Banchereau J, Oh S
Mucosal immunology 2013 May;6(3):626-38
Mucosal immunology 2013 May;6(3):626-38
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Alonzo F 3rd, Kozhaya L, Rawlings SA, Reyes-Robles T, DuMont AL, Myszka DG, Landau NR, Unutmaz D, Torres VJ
Nature 2013 Jan 3;493(7430):51-5
Nature 2013 Jan 3;493(7430):51-5
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
International journal of cancer 2010 Jul 15;127(2):332-44
International journal of cancer 2010 Jul 15;127(2):332-44
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadière C, Mami-Chouaib F
Cancer research 2009 Aug 1;69(15):6249-55
Cancer research 2009 Aug 1;69(15):6249-55
Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
Bolovan-Fritts CA, Spector SA
Blood 2008 Jan 1;111(1):175-82
Blood 2008 Jan 1;111(1):175-82
Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
International journal of cancer 2008 Feb 15;122(4):769-76
International journal of cancer 2008 Feb 15;122(4):769-76
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
Cytokine 2006 Dec;36(5-6):237-44
Cytokine 2006 Dec;36(5-6):237-44
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
Cellular signalling 2005 Mar;17(3):355-63
Cellular signalling 2005 Mar;17(3):355-63
Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsić B
Journal of neuroimmunology 2005 Jun;163(1-2):128-34
Journal of neuroimmunology 2005 Jun;163(1-2):128-34
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Lim HW, Hillsamer P, Kim CH
The Journal of clinical investigation 2004 Dec;114(11):1640-9
The Journal of clinical investigation 2004 Dec;114(11):1640-9
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Härtlein M, Kron M, Yang D, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ
The Journal of experimental medicine 2002 Sep 16;196(6):781-91
The Journal of experimental medicine 2002 Sep 16;196(6):781-91
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
The Journal of biological chemistry 1999 Apr 2;274(14):9617-26
The Journal of biological chemistry 1999 Apr 2;274(14):9617-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCR5 in Human Dendritic Cells. CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL3/MIP-1 alpha and Neutraliza-tion by Human CCR5 Antibody. Recombinant Human CCL3/MIP-1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP-1 alpha (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND50 is typically 5-20 µg/mL.
