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LearnPA5-78949
antibody from Invitrogen Antibodies
Targeting: CCR5
CC-CKR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-78949 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells.
Neutrophils contribute to spontaneous resolution of liver inflammation and fibrosis via microRNA-223.
Mladinich MC, Conde JN, Schutt WR, Sohn SY, Mackow ER
mBio 2021 Aug 31;12(4):e0196221
mBio 2021 Aug 31;12(4):e0196221
Neutrophils contribute to spontaneous resolution of liver inflammation and fibrosis via microRNA-223.
Calvente CJ, Tameda M, Johnson CD, Del Pilar H, Lin YC, Adronikou N, De Mollerat Du Jeu X, Llorente C, Boyer J, Feldstein AE
The Journal of clinical investigation 2019 Jul 11;129(10):4091-4109
The Journal of clinical investigation 2019 Jul 11;129(10):4091-4109
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCR5 in mouse liver tissue lysate using 50 µg per well. Sample was incubated with CCR5 (Product # PA5-78949) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCR5 in Lane 1: JURKAT cell lysate, Lane 2: COLO320 cell lysate. Sample was incubated with CCR5 polyclonal antibody (Product # PA5-78949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCR5 in cell lysates, JURKAT Cell Lysate (Lane 1) and COLO320 Cell Lysate (Lane 2), using a CCR5 Polyclonal Antibody (Product # PA5-78949).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCR5 in mouse liver tissue (50 μg). Samples were incubated in CCR5 Polyclonal Antibody (Product # PA5-78949, dilution 0.5 μg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CCR5 on paraffin-embedded human rectal cancer tissue. Sample was incubated with CCR5 polyclonal antibody (Product# PA5-78949).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG 4 Knockout of the CCL5 receptors CCR3 and CCR5 limits ZIKV infection of hBMECs. (A) Puromycin-selected CCR3-KO and CCR5-KO hBMECs were evaluated for CCR3 or CCR5 protein expression, compared to GAPDH, by Western blotting. (B and C) WT, CRISPR control, and CCL5-KO hBMECs were ZIKV infected (MOI of 10), and ZIKV infection was detected by ZIKV antigen-positive cells at 18 hpi (B) and 3 dpi (C). (D and E) At 3 dpi, supernatants were assessed for viral titers (D), and viability was assessed via CyQuant uptake (E). Asterisks indicate statistical significance (*, P < 0.05; ***, P < 0.001). Experiments were performed at least 3 times with similar results.
