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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005699-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005699-M01, RRID:AB_2171873
- Product name
- Mouse Monoclonal PSMB10/MECL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PSMB10 - proteasome (prosome, macropain) subunit, beta type, 10 (3F8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Endoplasmic reticulum stress activates autophagy but not the proteasome in neuronal cells: implications for Alzheimer's disease.
Nijholt DA, de Graaf TR, van Haastert ES, Oliveira AO, Berkers CR, Zwart R, Ovaa H, Baas F, Hoozemans JJ, Scheper W
Cell death and differentiation 2011 Jun;18(6):1071-81
Cell death and differentiation 2011 Jun;18(6):1071-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Detection limit for recombinant GST tagged PSMB10 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Analysis of monoclonal antibody to PSMB10 on HeLa cell. Antibody concentration 10 ug/ml.
