Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 15976-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLKPALEPR GGFSFENCQR NASLERVLPG LKVPHARKTG TTIAGLVFQD GVILGADTRA TNDSVVADKS CEKIHFIAPK IYCCGAGVAA DAEMTTRMVA SKMELHALST GREPRVATVT RILRQTLFRY QGHVGASLIV GGVDLTGPQL YGVHPHGSYS RLPFTALGSG QDAALAVLED RFQPNMTLEA AQGLLVEAVT AGILGDLGSG GNVDACVITK TGAKLLRTLS SPTEPVKRSG RYHFVPGTTA VLTQTVKPLT LELVEETVQA MEVE (1-273 aa encoded by BC017198)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15976-1-AP (PSMB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human liver tissue were subjected to SDS PAGE followed by western blot with 15976-1-AP (PSMB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 15976-1-AP (PSMB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 15976-1-AP (PSMB10 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 15976-1-AP (PSMB10 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 15976-1-AP) (PSMB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
