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antibody from Invitrogen Antibodies
Targeting: WDR62
C19orf14, DKFZP434J046, FLJ33298, MCPH2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-31552 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-WDR62 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: NIH-3T3. Predicted reactivity: Mouse (100%), Rat (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Wdr62 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Wdr62 polyclonal antibody (Product # PA5-31552) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WDR62 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WDR62 Polyclonal Antibody (Product # PA5-31552) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of WDR62 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: WDR62 Polyclonal Antibody (Product # PA5-31552) diluted at 1:5000. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of WDR62 was performed in frozen-sectioned rat E13.5 brain tissue using WDR62 Polyclonal Antibody (Product # PA5-31552) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WDR62 Polyclonal Antibody detects Wdr62 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. WDR62 Polyclonal Antibody (Product # PA5-31552) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WDR62 Polyclonal Antibody detects Wdr62 protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. WDR62 Polyclonal Antibody (Product # PA5-31552) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WDR62 Polyclonal Antibody detects Wdr62 protein at nucleus on Ca922 oral cancer xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 oral cancer xenograft. WDR62 Polyclonal Antibody (Product # PA5-31552) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
