21499-1-AP

antibody from Invitrogen Antibodies

Targeting: SH3D19 DKFZp434D0215, EBP, Eve-1, EVE1, Kryn, SH3P19

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21499-1-AP

Provider

Invitrogen Antibodies

Product name

SH3D19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VLVMLKQTE NNYLECQKGE DTGRVHLSQM KIITPLDEHL RSRPNDPSHA QKPVDSGAPH AVVLHDFPAE QVDDLNLTSG EIVYLLEKID TDWYRGNCRN QIGIFPANYV KVIIDIPEGG NGKRECVSSH CVKGSRCVAR FEYIGEQKDE LSFSEGEIII LKEYVNEEWA RGEVRGRTGI FPLNFVEPVE DYPTSGANVL STKVPLKTKK EDSGSNSQVN SLPAEWCEAL HSFTAETSDD LSFKRGDRIQ ILERLDSDWC RGRLQDREGI FPAVFVRPCP AEAKSMLAIV PKGRKAKALY DFRGENEDEL SFKAGDIITE LESVDDDWMS GELMGKSGIF PKNYIQFLQI S (411-790 aa encoded by BC108890)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).