PA5-60938

antibody from Invitrogen Antibodies

Targeting: POLR2I hRPB14.5, RPB9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60938

Provider

Invitrogen Antibodies

Product name

POLR2I Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PDGTYEPGFV GIRFCQECNN MLYPKEDKEN RILLYACRNC DYQQEADNSC IYVNKITHEV DELTQIIADV SQDPTLPRTE DHPCQKCGHK EAVFFQSHSA

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLR2I in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a POLR2I Polyclonal Antibody (Product # PA5-60938).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLR2I in human cell line HEK 293 using a POLR2I Polyclonal Antibody (Product # PA5-60938).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POLR2I in mouse cell line NIH-3T3 and rat cell line NBT-II using a POLR2I Polyclonal Antibody (Product # PA5-60938).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of POLR2I in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a POLR2I Polyclonal Antibody (Product # PA5-60938).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of POLR2I in human urinary bladder tissue shows moderate nuclear positivity in urothelial cells. Samples were probed using a POLR2I Polyclonal Antibody (Product # PA5-60938).