Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF756 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TSG Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse TSG in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin.
Tanno T, Bhanu NV, Oneal PA, Goh SH, Staker P, Lee YT, Moroney JW, Reed CH, Luban NL, Wang RH, Eling TE, Childs R, Ganz T, Leitman SF, Fucharoen S, Miller JL
Nature medicine 2007 Sep;13(9):1096-101
Nature medicine 2007 Sep;13(9):1096-101
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TSG in Mouse Embryo. TSG was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TSG in Mouse Embryo. TSG was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse TSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF756) overnight at 4 °C. Tissue was stained (red) and counter-stained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.