LS-C377134

antibody from LSBio

Targeting: TBCB CG22, CKAP1, CKAPI

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C377134

Provider

LSBio

Product name

TBCB / CKAP1 Antibody LS-C377134

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating TBCB in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TBCB Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TBCB Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TBCB in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TBCB Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TBCB Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating TBCB in Hela whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of TBCB Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: TBCB Antibody (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)