ANC-004-200UL

antibody from Invitrogen Antibodies

Targeting: CHRNA4 BFNC, EBN, EBN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANC-004-200UL

Provider

Invitrogen Antibodies

Product name

Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 (extracellular) Blocking Peptide (#BLP-NC004).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplex staining of nAChR alpha 4 and nAChR beta 2 in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:300) and Anti-Nicotinic Acetylcholine Receptor beta 2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody (#ANC-012-AR), (1:60).A. nAChR alpha 4 staining (green). B. Same section stained for nAChR beta 2 (red). C. Merge of the two images reveals several cells expressing both receptors (arrows). Nuclei are stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of nAChR alpha 4 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody (#ANC-004), (1:200). A. Expression of nAChR alpha 4 (green) was visualized in rat cerebellum. Purkinje cells were positive for nAChR alpha 4 (arrows). B. The same section was stained for Parvalbumin (red) and counterstained (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection ofnAChR alpha 4by indirect flow cytometry in live intacthumanJurkatT-cell leukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (extracellular) Antibody   (#ANC-004), (2.5μg) + goat- Anti-rabbit-FITC.