15144-1-AP

antibody from Invitrogen Antibodies

Targeting: PPIL1 CYPL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15144-1-AP

Provider

Invitrogen Antibodies

Product name

PPIL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAAIPPDSW QPPNVYLETS MGIIVLELYW KHAPKTCKNF AELARRGYYN GTKFHRIIKD FMIQGGDPTG TGRGGASIYG KQFEDELHPD LKFTGAGILA MANAGPDTNG SQFFVTLAPT QWLDGKHTIF GRVCQGIGMV NRVGMVETNS QDRPVDDVKI IKAYPSG (1-166 aa encoded by BC003048)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

1.05 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug.