21778-1-AP

antibody from Invitrogen Antibodies

Targeting: POLA2 FLJ21662

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21778-1-AP

Provider

Invitrogen Antibodies

Product name

POLA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LDCEEALIE KLVELCVQYG QNEEGMVGEL IAFCTSTHKV GLTSEILNSF EHEFLSKRLS KARHSTCKDS GHAGARDIVS IQELIEVEEE EEILLNSYTT PSKGSQKRAI STPETPLTKR SVSTRSPHQL LSPSSFSPSA TPSQKYNSRS NRGEVVTSFG LAQGVSWSGR GGAGNISLKV LGCPEALTGS YKSMFQKLPD IREVLTCKIE ELGSELKEHY KIEAFTPLLA PAQEPVTLLG QIGCDSNGKL NNKSVILEGD (17-275 aa encoded by BC001347)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).