210-301-C32S

antibody from Rockland Immunochemicals, Inc.

Targeting: ESRP2 FLJ21918, RBM35B

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

210-301-C32S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#210-301-C32S, RRID:AB_10894685

Product name

Anti-Esrp-2 (MOUSE) Monoclonal Antibody - 210-301-C32S

Antibody type

Monoclonal

Vial size

25 µl

References

Submitted references

Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer.

Heilmann K, Toth R, Bossmann C, Klimo K, Plass C, Gerhauser C
Oncogene 2017 Nov 16;36(46):6446-6461

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.

Validation comment

Western Blot