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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [5]
- Immunohistochemistry [4]
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- Product number
- GTX102326 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102326, RRID:AB_1950166
- Product name
- EEF1A2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival.
Comparative Proteomic and Transcriptomic Analysis of Follistatin-Induced Skeletal Muscle Hypertrophy.
Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin.
In vivo characterization of the role of tissue-specific translation elongation factor 1A2 in protein synthesis reveals insights into muscle atrophy.
Losada A, Muñoz-Alonso MJ, Martínez-Díez M, Gago F, Domínguez JM, Martínez-Leal JF, Galmarini CM
British journal of cancer 2018 Nov;119(11):1410-1420
British journal of cancer 2018 Nov;119(11):1410-1420
Comparative Proteomic and Transcriptomic Analysis of Follistatin-Induced Skeletal Muscle Hypertrophy.
Barbé C, Bray F, Gueugneau M, Devassine S, Lause P, Tokarski C, Rolando C, Thissen JP
Journal of proteome research 2017 Oct 6;16(10):3477-3490
Journal of proteome research 2017 Oct 6;16(10):3477-3490
Translation Elongation Factor eEF1A2 is a Novel Anticancer Target for the Marine Natural Product Plitidepsin.
Losada A, Muñoz-Alonso MJ, García C, Sánchez-Murcia PA, Martínez-Leal JF, Domínguez JM, Lillo MP, Gago F, Galmarini CM
Scientific reports 2016 Oct 7;6:35100
Scientific reports 2016 Oct 7;6:35100
In vivo characterization of the role of tissue-specific translation elongation factor 1A2 in protein synthesis reveals insights into muscle atrophy.
Doig J, Griffiths LA, Peberdy D, Dharmasaroja P, Vera M, Davies FJ, Newbery HJ, Brownstein D, Abbott CM
The FEBS journal 2013 Dec;280(24):6528-40
The FEBS journal 2013 Dec;280(24):6528-40
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX102326 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) C: Molt-4 (GTX27912) D: Raji 10% SDS PAGE GTX102326 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1A2 antibody detects EEF1A2 protein by western blot analysis.A. 50 ?g mouse muscle lysate/extract10% SDS-PAGEEEF1A2 antibody (GTX102326) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody (GTX102326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1A2 antibody detects EEF1A2 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1A2 antibody (GTX102326) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1A2 antibody detects EEF1A2 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. EEF1A2 antibody (GTX102326) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1A2 antibody detects EEF1A2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. EEF1A2 antibody (GTX102326) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EEF1A2 antibody detects EEF1A2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EEF1A2 antibody (GTX102326) diluted at 1:500.
