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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-58330 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMIE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RTRKEIEARY LQRKAAKMYT DKLETVPPLN ELTEVPGEDK KKKK
- Concentration
- 0.2 mg/mL
Submitted references Hair cell α9α10 nicotinic acetylcholine receptor functional expression regulated by ligand binding and deafness gene products.
Gu S, Knowland D, Matta JA, O'Carroll ML, Davini WB, Dhara M, Kweon HJ, Bredt DS
Proceedings of the National Academy of Sciences of the United States of America 2020 Sep 29;117(39):24534-24544
Proceedings of the National Academy of Sciences of the United States of America 2020 Sep 29;117(39):24534-24544
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TMIE in human hippocampus shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a TMIE Polyclonal Antibody (Product # PA5-58330).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5. TMIE regulates a9a10 gating as a receptor auxiliary subunit. ( A - D ) Patch-clamp analysis of cells transfected with a9a10 + ChAT (black) or a9a10 + TMIE (red) and preincubated with 5 mM ACh for 24 h. ACh (100 uM) was applied for 2 s or 2 ms to assess channel desensitization or deactivation, respectively. Representative traces followed by summary graphs are shown (mean +- SEM, n as indicated, ** P
