22117-1-AP

antibody from Invitrogen Antibodies

Targeting: PPP1R12A M130, MBS, MYPT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22117-1-AP

Provider

Invitrogen Antibodies

Product name

MYPT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AEKTIGRSR STRTREQENE EKEKEEKEKQ DKEKQEEKKE SETSREDEYK QKYSRTYDET YQRYRPVSTS SSTTPSSSLS TMSSSLYASS QLNRPNSLVG ITSAYSRGIT KENEREGEKR EEEKEGEDKS QPKSIRERRR PREKRRSTGV SFWTQDSDEN EQEQQSDTEE GSNKKETQTD SISRYETSST SAGDRYDSLL GRSGSYSYLE ERKPYSSRLE KDDSTDFKKL YEQILAENEK LKAQLHDTNM ELTDLKLQLE KATQRQERFA DRSLLE (707-981 aa encoded by BC111752)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.64 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of MYPT1 antibody (22117-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYPT1 transfected HEK-293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 22117-1-AP (MYPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle using 22117-1-AP (MYPT1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 22117-1-AP ( MYPT1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 22117-1-AP ( MYPT1 antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MYPT1 (IP:22117-1-AP, 4ug; Detection:22117-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.