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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- NBP2-22425 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ADAM22 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects the extracellular domain of ADAM22 approx 90kDa. Does not cross-react with ADAM11. Weakly reactive in human samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins.
Richter KN, Wildhagen H, Helm MS, Ußling JE, Schikorski T, Rizzoli SO
Scientific reports 2018 Oct 4;8(1):14838
Scientific reports 2018 Oct 4;8(1):14838
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ADAM22 Antibody (S57-2) [NBP2-22425] - analysis of Rat brain lysates showing detection of ADAM22 protein using Mouse Anti-ADAM22 Monoclonal Antibody, Clone S57-2 . Primary Antibody: Mouse Anti-ADAM22 Monoclonal Antibody at 1:250, 1:500, and 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ADAM22 Antibody (S57-2) [NBP2-22425] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ADAM22 (extracellular) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.
