Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-90592 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: AEREFRGLGD CLVKIYKSDG IKGLYQGFNV SVQGIIIYRA AYFGIYDTAK GMLPDPKNTH IVISWMIAQT VTAVAGLTSY PFDTVRRRMM MQSGRKGTDI M; Positive Samples: 293T, LO2, Mouse brain, Mouse heart, Mouse kidney, Rat kidney, Rat brain; Cellular Location: Mitochondrion inner membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.81 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC25A5 in extracts of various cell lines using SLC25A5 Polyclonal Antibody (Product # PA5-90592) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SLC25A5 was performed in HeLa cells using SLC25A5 Polyclonal Antibody (Product # PA5-90592).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A5 in paraffin-embedded rat heart using SLC25A5 Polyclonal Antibody (Product # PA5-90592) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A5 in paraffin-embedded mouse heart using SLC25A5 Polyclonal Antibody (Product # PA5-90592) at a dilution of 1:100.