PAB28637

antibody from Abnova Corporation

Targeting: VPREB3 8HS20

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28637

Provider

Abnova Corporation

Product name

VPREB3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant VPREB3.

Antigen sequence

QLDALLVFPGQVAQLSCTLSPQHVTIRDYGVSWYQ
QRAGSAPRYLLYYRSEEDHHRPADIPDRFSAAKDE
AHNACVLTISPVQPEDDADYYCSVGYGFSP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with VPREB3 polyclonal antibody (Cat # PAB28637).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with VPREB3 polyclonal antibody (Cat # PAB28637) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with VPREB3 polyclonal antibody (Cat # PAB28637) shows strong cytoplasmic positivity in reaction center cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)