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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- GTX106105 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX106105, RRID:AB_1952006
- Product name
- Sorcin antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE GTX106105 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:MOLT4(GTX27912)15% SDS PAGEGTX106105 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Sorcin knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Sorcin antibody [N1C3] (GTX106105) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sorcin antibody [N1C3] immunoprecipitates Sorcin protein in IP experiments.IP samples: HeLa whole cell extractA. 40 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Sorcin protein by 4 £gg Sorcin antibody [N1C3] (GTX106105)12 % SDS-PAGEThe immunoprecipitated Sorcin protein was detected by Sorcin antibody [N1C3] (GTX106105) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
