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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF5797 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat CD39L1/ENTPD2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat CD39L1/ENTPD2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) CD39, rmCD39L2, and rmCD39L3 is observed.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O55026- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Role of P2X(4) Receptor in Mouse Voiding Function.
Expression and distribution of ectonucleotidases in mouse urinary bladder.
Yu W, Hill WG, Robson SC, Zeidel ML
Scientific reports 2018 Jan 30;8(1):1838
Scientific reports 2018 Jan 30;8(1):1838
Expression and distribution of ectonucleotidases in mouse urinary bladder.
Yu W, Robson SC, Hill WG
PloS one 2011 Apr 14;6(4):e18704
PloS one 2011 Apr 14;6(4):e18704
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse and Rat CD39L1/ENTPD2 by Western Blot. Western blot shows lysates of mouse salivary gland tissue and rat placenta tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
