AMAb91603

antibody from Atlas Antibodies

Targeting: SETD2 FLJ23184, HIF-1, HYPB, KIAA1732, KMT3A

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91603

Provider

Atlas Antibodies

Product name

Anti-SETD2

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

PHHPFAGYPPGYPMQAYVDPSNPN


Epitope

Binds to an epitope located within the peptide sequence - as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL9956

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using AMAb91603 antibody. Corresponding SETD2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows very weak positivity in myocytes as expected.