Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB3214 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3214, RRID:AB_10553560
- Product name
- PPIF monoclonal antibody, clone AT1F5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PPIF.
- Isotype
- IgG
- Antibody clone number
- AT1F5
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Construction of a high-resolution physical map of the chromosome 10q22-q23 dilated cardiomyopathy locus and analysis of candidate genes.
The cyclophilin multigene family of peptidyl-prolyl isomerases. Characterization of three separate human isoforms.
Bowles KR, Abraham SE, Brugada R, Zintz C, Comeaux J, Sorajja D, Tsubata S, Li H, Brandon L, Gibbs RA, Scherer SE, Bowles NE, Towbin JA
Genomics 2000 Jul 15;67(2):109-27
Genomics 2000 Jul 15;67(2):109-27
The cyclophilin multigene family of peptidyl-prolyl isomerases. Characterization of three separate human isoforms.
Bergsma DJ, Eder C, Gross M, Kersten H, Sylvester D, Appelbaum E, Cusimano D, Livi GP, McLaughlin MM, Kasyan K
The Journal of biological chemistry 1991 Dec 5;266(34):23204-14
The Journal of biological chemistry 1991 Dec 5;266(34):23204-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
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- Experimental details
- Cell lysates of 293T and HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with PPIF monoclonal antibody, clone AT1F5 (Cat # MAB3214 ; 1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.