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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP1-28608 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28608, RRID:AB_1853215
- Product name
- Mouse Monoclonal Cyclophilin-F Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Detection of Cyclophilin-F in HeLa Whole Cell Lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclophilin-F Antibody (1F5) [NBP1-28608] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIF antibody at a dilution of (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HepG2 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cyclophilin-F Antibody (1F5) [NBP1-28608] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
