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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31061 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclophilin F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, mouse heart, rat heart.
- Concentration
- 0.67 mg/mL
Submitted references Therapeutic efficacy of cyclosporin A against spinal cord injury in rats with hyperglycemia.
Chen ZR, Ma Y, Guo HH, Lu ZD, Jin QH
Molecular medicine reports 2018 Mar;17(3):4369-4375
Molecular medicine reports 2018 Mar;17(3):4369-4375
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPIF using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a PPIF polyclonal antibody (Product # PA5-31061) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclophilin F was performed by separating 50 µg of mouse tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cyclophilin F Polyclonal Antibody (Product # PA5-31061) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclophilin F was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cyclophilin F Polyclonal Antibody (Product # PA5-31061) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclophilin F was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Cyclophilin F Polyclonal Antibody (Product # PA5-31061) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PPIF in methanol-fixed A431 cells using a PPIF polyclonal antibody (Product # PA5-31061) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF (Product # PA5-31061) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
