Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX105792 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105792, RRID:AB_1950411
- Product name
- Glyoxalase I antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 12% SDS PAGE GTX105792 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30?g whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)12% SDS PAGEGTX105792 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glyoxalase I antibody [N1C3] detects Glyoxalase I protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract12% SDS-PAGEGlyoxalase I antibody [N1C3] (GTX105792) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.A. 30 £gg A431 whole cell lysate/extract B. 30 £gg HeLa whole cell lysate/extract C. 30 £gg HepG2 whole cell lysate/extract12 % SDS-PAGEGlyoxalase I antibody (GTX105792) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GLO1(GTX105792) antibody at 1:100 dilution.
