Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-44216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: TMLRVKDPKK SLDFYTRVLG MTLIQKCDFP IMKFSLYFLA YEDKNDIPKE Sequence homology: Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 86%; Zebrafish: 86%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 cell lysate using an anti-GLO1 polyclonal antibody (Product # PA5-44216).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GLO1 Polyclonal Antibody (Product # PA5-44216) and a 21 kDa band corresponding to GLO1 was observed in Mouse Liver and Rat Liver and not in Mouse Skeletal Muscle and Mouse Spleen tissue lysates which is reported to be negative. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Spleen (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).