Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-49942 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49942, RRID:AB_10012132
- Product name
- Rabbit Polyclonal WWP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: WWP2 Antibody [NBP1-49942] - Detection of Human and Mouse WWP2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-WWP2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. WWP2 was also immunoprecipitated by rabbit anti-WWP2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated WWP2 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 second (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WWP2 Antibody [NBP1-49942] - Detection of mouse WWP2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug), and HeLa (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for IP at 6 ug per reaction. WWP2 was also immunoprecipitated by another rabbit anti-WWP2 antibody. For blotting immunoprecipitated WWP2, NBP1-49942 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.