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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-88379 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88379, RRID:AB_11028271
- Product name
- Rabbit Polyclonal CDC42EP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CDC42EP1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC42EP1 Antibody [NBP1-88379] - HeLa whole cell lysate. Lanes 1 and 4: HeLa cells transfected with control non-targeting siRNA. Lanes 2 and 3: HeLa cells transfected with two different siRNA-targeting Cdc42EP1. Blot probed with cdc42ep1 antibody and reprobed with GAPDH as loading control. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CDC42EP1 Antibody [NBP1-88379] - Lanes 1 and 5: Marker. Lanes 2-4 HeLa cell input controls. Lane 6: IP using 2 mg of Control Rabbit IgG cell lysate. Lane 8: IP using 2 mg Cdc42EP1 cell lysate and Betheyl antibody A302-381A. Lane 10: IP using 2 mg Cdc42EP1 cell lysate and Novus antibody NBP1-88379. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDC42EP1 Antibody [NBP1-88379] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
