66097-1-IG

antibody from Invitrogen Antibodies

Targeting: ORM2 AGP-B, AGP-B', AGP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

66097-1-IG

Provider

Invitrogen Antibodies

Product name

ORM1/2 Monoclonal Antibody (5F2H7)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

Antibody clone number

5F2H7

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

25µg human testis tissue was subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Untreated and PNGase F-treated lysates of HuH-7 cells were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

0.8µL human plasma tissue were subjected to SDS PAGE followed by western blot with 66097-1-IG (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66097-1-IG (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( ) fixed HepG2 cells using 66097-1-IG (ORM1/2 antibody) at dilution of 1:25 and N/A.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66097-1-IG (ORM1/2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66097-1-IG (ORM1/2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human ORM1/2 (Product # 66097-1-IG, Clone:5F2H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).