Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31499 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF571 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ZNF571 Polyclonal Antibody (Product # PA5-31499). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 7.5% SDS PAGE. ZNF571 Polyclonal Antibody (Product # PA5-31499) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF571 Polyclonal Antibody detects ZNF571 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF571 protein stained by ZNF571 Polyclonal Antibody (Product # PA5-31499) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ZNF571 (Product # PA5-31499) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.