PAB20735

antibody from Abnova Corporation

Targeting: RNF130 G1RZFP, GOLIATH, GP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20735

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20735, RRID:AB_10987294

Product name

RNF130 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RNF130.

Antigen sequence

PWLSEHCTCPMCKLNILKALGIVPNLPCTDNVAFD
MERLTRTQAVNRRSA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RNF130 polyclonal antibody (Cat # PAB20735) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RNF130 polyclonal antibody (Cat # PAB20735) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with RNF130 polyclonal antibody (Cat # PAB20735) shows strong cytoplasmic positivity in cells in tubules at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)