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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP09123PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09123PU-S, RRID:AB_2035957
- Product name
- anti SIX3
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of Mouse Six3 protein
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Guinea Pig
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 0.65 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Protein A purified anti-Six3 antibody shows detection of Six3 in whole cell lysate. The band marked corresponds to Six3 at an approximate molecular weight of 37Kda. The primary antibody was used at a 1:1000 dilution. Personal Communication, N. Johnson, St Jude Children's Research Hospital, Memphis, TN.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy using Protein A purified anti-Six3 antibody shows detection of Six3 in various E14.5 mouse embryonic tissues. Tissue was fixed using 4% PFA. The primary antibody was used at a 1:200 dilution. Personal Communication, A. Lavado, St Jude Children's Research Hospital, Memphis, TN.
