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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- GTX628898 - Provider product page
- Provider
- GeneTex
- Product name
- SIX2 antibody [EPR4824]
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
Submitted references Heterogeneous Cadherin Expression and Multicellular Aggregate Dynamics in Ovarian Cancer Dissemination.
Klymenko Y, Johnson J, Bos B, Lombard R, Campbell L, Loughran E, Stack MS
Neoplasia (New York, N.Y.) 2017 Jul;19(7):549-563
Neoplasia (New York, N.Y.) 2017 Jul;19(7):549-563
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFP antibody detects RFP protein by Western blot analysis.A. 30 µg 293T whole cell extractB. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGERFP antibody (GTX628898) dilution: 1:10000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 £gg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 £gg of RFP tag antibody (GTX628898) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628898) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.
