H00010736-M01

antibody from Novus Biologicals

Targeting: SIX2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00010736-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00010736-M01, RRID:AB_2189169

Product name

Mouse Monoclonal SIX2 Antibody

Antibody type

Monoclonal

Description

IgG purified. SIX2 - sine oculis homeobox homolog 2 (Drosophila)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.

Hariharan K, Reinke P, Kurtz A
Methods in molecular biology (Clifton, N.J.) 2019;1926:103-115

---

Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.

Monteiro Carvalho Mori da Cunha MG, Zia S, Oliveira Arcolino F, Carlon MS, Beckmann DV, Pippi NL, Luhers Graça D, Levtchenko E, Deprest J, Toelen J
PloS one 2015;10(8):e0136145

---

ROBO2 restricts the nephrogenic field and regulates Wolffian duct-nephrogenic cord separation.

Wainwright EN, Wilhelm D, Combes AN, Little MH, Koopman P
Developmental biology 2015 Aug 15;404(2):88-102

---

Fat4/Dchs1 signaling between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branching.

Mao Y, Francis-West P, Irvine KD
Development (Cambridge, England) 2015 Aug 1;142(15):2574-85

---

Mdm2 is required for maintenance of the nephrogenic niche.

Hilliard SA, Yao X, El-Dahr SS
Developmental biology 2014 Mar 1;387(1):1-14

---

Identification of human nephron progenitors capable of generation of kidney structures and functional repair of chronic renal disease.

Harari-Steinberg O, Metsuyanim S, Omer D, Gnatek Y, Gershon R, Pri-Chen S, Ozdemir DD, Lerenthal Y, Noiman T, Ben-Hur H, Vaknin Z, Schneider DF, Aronow BJ, Goldstein RS, Hohenstein P, Dekel B
EMBO molecular medicine 2013 Oct;5(10):1556-68

---

Transcription coactivator Eya2 is a critical regulator of physiological hypertrophy.

Lee SH, Kim J, Ryu JY, Lee S, Yang DK, Jeong D, Kim J, Lee SH, Kim JM, Hajjar RJ, Park WJ
Journal of molecular and cellular cardiology 2012 Mar;52(3):718-26

---

SIX2 and CITED1, markers of nephronic progenitor self-renewal, remain active in primitive elements of Wilms' tumor.

Murphy AJ, Pierce J, de Caestecker C, Taylor C, Anderson JR, Perantoni AO, de Caestecker MP, Lovvorn HN 3rd
Journal of pediatric surgery 2012 Jun;47(6):1239-49

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.