11562-1-AP

antibody from Invitrogen Antibodies

Targeting: SIX2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11562-1-AP

Provider

Invitrogen Antibodies

Product name

SIX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is a rabbit polyclonal antibody raised against full length SIX2 of human origin.

Concentration

0.52 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP (SIX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11562-1-AP ( SIX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result from Fogelgren B, PMID:18570229, "Misexpression of Six2 is associated with heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice.".

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 11562-1-AP (SIX2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 11562-1-AP (SIX2 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SIX2 (Product # 11562-1-AP) with HEK-293 cells lysate 1,320 µg. IP: 11562-1-AP, 4 µg; Detection: 11562-1-AP 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SIX2 (IP:11562-1-AP, 3ug; Detection:11562-1-AP 1:500) with HEK-293 cells lysate 2700ug.