66347-1-IG

antibody from Invitrogen Antibodies

Targeting: SIX2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66347-1-IG

Provider

Invitrogen Antibodies

Product name

SIX2 Monoclonal Antibody (1F9G11)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MSMLPTFGF TQEQVACVCE VLQQGGNIER LGRFLWSLPA CEHLHKNESV LKAKAVVAFH RGNFRELYKI LESHQFSPHN HAKLQQLWLK AHYIEAEKLR GRPLGAVGKY RVRRKFPLPR SIWDGEETSY CFKEKSRSVL REWYAHNPYP SPREKRELTE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN NENSNSNSHN PLNGSGKSVL GSSEDEKTPS GTPDHSSSSP ALLLSPPPPG LPSLHSLGHP PGPSAVPVPV PGGGGADPLQ HHHGLQDSIL NPMSANLVDL GS (1-291 aa encoded by BC024033)

Reactivity

Human, Mouse, Rat, Canine, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

1F9G11

Vial size

150 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-IG (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 66347-1-IG (SIX2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).